About Vitamin A Analysis and Units

Vitamin A analysis is based on extraction; HPLC device is used for the final result. Results are generally given in IU / kg.

In addition to the most basic unit IU / kg; it is possible to use other vitamin units. In this sense, units such as Vitamin A acetate and Vitamin A palmitate are also encountered.

Vitamin A analysis results can be given in either acetate or palmitate. Regardless of the unit of results expressed; the same pretreatment is applied to the sample. The saponified sample is made available to the last device. Calibration curve is used in which vitamin A results are desired and analysis results are obtained. As a second way, the unit conversions can be performed and the results can be converted to the desired Vitamin A result. When separate analyzes for acetate and palmitate are performed on standards; The results of the unit conversion were obtained and controls were made.

The following table shows the most commonly used Vitamin A units and conversion factors.

Table of Vitamin A Units

Retinol activity equivalent (RAE) and vitamin A activity (IU) conversion factors of the amount of vitamin A source material
Source Material (1 mcg)	Amount of Vitamin A (mcg RAE)	Vitamin A activity (IU)
all-trans beta karoten (beta karoten)	0.50	1.67
all-trans retinol (1 mcg RAE)	1.00	3.33
all-trans retinil asetat (vitamin A asetat)	0.87	2.94
all-trans retinil palmitat (vitamin A palmitat)	0.55	1.82

Table Vitamin A Units The retinol activity equivalent (RAE) and vitamin A activity (IU) conversion factors of the amount of vitamin A source material.
Improper preparation of premix for vitamin A analysis
Failure to properly mix premix into flour sample
- Premix and sample storage conditions (Vitamin A is particularly affected by light and air)